表达柔嫩艾美耳球虫HMGB1基因质粒DNA的免疫效果分析

doi:10.11843/j.issn.0366-6964.2016.02.019

Abstract

Abstract:

This experiment was conducted to assess the immuno-efficacy of the eukaryotic plasmid.The high mobility group box1 gene from Eimeria tenella was inserted into expression vector pcDNA3.1(-)/myc-His A，and then constructed the eukaryotic expression recombinant plasmid pcDNA3.1(-)/myc-EtHMGB1 and expressed transiently the objective protein in Hela cells.Seven experimental groups including pcDNA3.1(-)/myc-EtHMGB1 immunization group，pcDNA3.1(-)/myc-His A immunization group，recombinant antigen with adjuvant immunization group，pcDNA3.1(-)/myc-EtHMGB1 with recombinant antigen prime-boost immunization group，FCA adjuvant immunization group，unimmunized and challenged group and unimmunized and unchallenged group were designed.Chickens were vaccinated at the age of 14 and 21 days.All birds except the unchallenged control group were challenged orally with 1×10⁴E.tenella sporulated oocysts at 28 days.Gain of body weight，fecal oocyst output and lesion scores were assessed as measures of protective immunity.The results showed that the plasmid pcDNA3.1(-)/myc-EtHMGB1 was successfully constructed，and the transient expression product of EtHMGB1 could be detected by IFA and Western blot.Challenge experiments demonstrated that pcDNA3.1(-)/myc-EtHMGB1 immunization，recombinant antigen with adjuvant immunization and pcDNA3.1(-)/myc-EtHMGB1 with recombinant antigen prime-boost immunization could all increase body weight gains and reduce oocyst excretion，but all three methods did not have an effect on cecal lesion.The prime-boost immunization strategy were superior to the other method based on the immune effects.These results suggest that EtHMGB1 could be proposed as an anti-apicomplexan vaccine candidate.

CLC Number:

S852.723

GU You-fang，LI Wen-chao，WANG Kai，JIN Er-hui，HU Qian-qian，LI Sheng-he，CHEN Hui-liang . Protective Immunity Induced by a Plasmid Encoding Eimeria tenella High Mobility Group box1 Gene against Homologous Challenge[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 354-360.

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Protective Immunity Induced by a Plasmid Encoding Eimeria tenella High Mobility Group box1 Gene against Homologous Challenge

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